annexin v-apc/7-aad apoptosis kit  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Cellular Oncology

Article Title: BCAA catabolism mediates POU2AF1 propionylation to enhance T-ALL development

doi: 10.1007/s13402-026-01201-w

Figure Lengend Snippet: BCAT1 regulates T-ALL cell fate decisions. ( A ) Representative flow cytometry plots of leukemia cells (GFP + ) in the peripheral blood of recipient mice 3 weeks after secondary transplantation. ( B ) Quantification of leukemia cells (GFP + ) in peripheral blood after secondary transplantation ( n = 6). ( C ) Representative images of the size of the liver, spleen and thymus from recipient mice post-secondary transplantation. ( D ) Quantification of the data shown in ( C ) ( n = 6). ( E ) Overall survival was determined for the leukemic mice shown in ( B ) ( n = 6). ( F ) Representative flow cytometry plots of leukemia cells (GFP + ) in the peripheral blood of recipient mice 3 weeks after tertiary transplantation. ( G ) Quantification of leukemia cells (GFP + ) in peripheral blood after tertiary transplantation ( n = 6). ( H ) Organ weight quantification of the liver, spleen and thymus from transplanted mice ( n = 4). ( I ) Overall survival was determined for the leukemic mice shown in ( G ) ( n = 10). ( J ) Representative flow cytometry plots showing early and late apoptosis of WT and KO T-ALL cells in peripheral blood after secondary transplantation. ( K ) Quantification of apoptotic populations from Panel ( J ) ( n = 3). ( L ) Flow cytometric analysis of the homing efficiency of WT and KO T-ALL cells to bone marrow 16 h post-injection. ( M ) Quantification of homing efficiency, shown in ( L ) ( n = 5). ( N ) Cell cycle status was determined in WT and KO T-ALL cells of the recipients. ( O )Quantification data of the phases of the cell cycle in Panel ( N ) ( n = 3). The data are presented as the means ± SDs. Student’s two-tailed unpaired t test (B, G, M) , log-rank test ( E , I ) and two-way ANOVA with Sidak’s multiple comparison test ( D , H , K , O ) were used for the comparison of statistical significance (*, P < 0.05; **, P < 0.01; and ***, P < 0.001)

Article Snippet: Apoptosis was assessed using an Annexin V-APC Apoptosis Detection Kit (MULTI SCIENCE, AP105) according to the manufacturer’s protocol.

Techniques: Flow Cytometry, Transplantation Assay, Injection, Two Tailed Test, Comparison